IP-10 ELISA Kit (Human)
Referência OKAG00045
Tamanho : 1plate:12x8-WellMicrostrips
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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| Product Format | 1 x 96-Well Plate or 12 x 8-Well Strips | ||||||||||||||||||||||
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | 4.5 hours | ||||||||||||||||||||||
| ELISA Kit Principle | The Aviva Human IP-10 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIP-10 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human IP-10 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. | ||||||||||||||||||||||
| ELISA Kit Range | 8-2000 pg/ml | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Cell lysates, sera and plasma | ||||||||||||||||||||||
| Sensitivity | 8 pg/mL | ||||||||||||||||||||||
| Specificity | The Human IP-10 ELISA is capable of recognizing both recombinant and naturally produced Human IP-10 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human: BCA-1, ENA-78, FGF-Basic, GCP-2, GROα, GROβ, GROγ, IFN-γ, IL-8 (77 aa), IL-8 (72 aa), MIG, I-TAC, MIP-1β, NAP-2, SDF-1α, SDF-1β, sRANK-Ligand • Murine: BLC, IP-10, I-TAC, MIG, SDF-1α, SDF-1β, TNF-α • Rat: GROβ, IP-10, KC | ||||||||||||||||||||||
| Assay Info | Quantitative Colorimentric Sandwich ELISA |
| Gene Symbol | CXCL10 |
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| Gene Full Name | C-X-C motif chemokine ligand 10 |
| Alias Symbols | 10 kDa interferon gamma-induced protein;C7;crg-2;C-X-C motif chemokine 10;gamma IP10;gIP-10;IFI10;INP10;interferon-inducible cytokine IP-10;IP-10;mob-1;protein 10 from interferon (gamma)-induced cell line;SCYB10;small inducible cytokine subfamily B (Cys-X-Cys), member 10;small-inducible cytokine B10. |
| NCBI Gene Id | 3627 |
| Protein Name | P02778 |
| Description of Target | C-X-C motif chemokine 10 |
| Uniprot ID | https://www.uniprot.org/uniprot/P02778 |
| Protein Accession # | NP_001556.2 |
| Nucleotide Accession # | NM_001565.3 |