Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Levamisole (hydrochloride) [16595-80-5]

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Referência HY-13666-10g

Tamanho : 10g

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Marca : MedChemExpress

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Telefone : +1 850 650 7790

Levamisole ((-)-Tetramisole) hydrochloride is an anthelmintic and immunomodulator belonging to a class of synthetic imidazothiazole derivatives. Levamisole hydrochloride has antiviral effects against HSV.

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Levamisole hydrochloride Chemical Structure

CAS No. : 16595-80-5

This product is a controlled substance and not for sale in your territory.

Based on 4 publication(s) in Google Scholar

Other Forms of Levamisole hydrochloride:

Levamisole In-stock
Levamisole-d₅ hydrochloride Obtenir un devis

Voir tous les produits spécifiques à Isoform Parasite:

Voir toutes les isoformes

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Voir tous les produits spécifiques à Isoform HSV:

Voir toutes les isoformes

HSV-1 HSV-2 HSV

Description

IC₅₀ & Target^[1]^[2]^[3]

Parasite

HSV-1

In Vitro

Levamisole suppresses the production of white blood cells, resulting in neutropenia and agranulocytosis. With the increasing use of levamisole as an adulterant, a number of these complications have been reported among cocaine users [1] [2]. Levamisole reversibly and noncompetitively inhibits most isoforms of alkaline phosphatase (e.g., human liver, bone, kidney, and spleen) except the intestinal and placental isoform [3]. It is thus used as an inhibitor along with substrate to reduce background alkaline phosphatase activity in biomedical assays involving detection signal amplification by intestinal alkaline phosphatase, for example in in situ hybridization or Western blot protocols. It is used to immobilize the nematode C. elegans on glass slides for imaging.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Essai clinique

Masse moléculaire

240.75

Formule

C₁₁H₁₃ClN₂S

CAS No.

16595-80-5

Appearance

Solid

Color

White to off-white

SMILES

15Cl.C12=N[C@@H](C3=CC=CC=C3)CN1CCS2

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Solvant et solubilité

In Vitro:

H₂O : 50 mg/mL (207.68 mM; Need ultrasonic)

DMSO : 15.5 mg/mL (64.38 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 12.5 mg/mL (51.92 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1537 mL	20.7684 mL	41.5369 mL
5 mM	0.8307 mL	4.1537 mL	8.3074 mL
10 mM	0.4154 mL	2.0768 mL	4.1537 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (5.19 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (5.19 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (5.19 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (415.37 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureté et documentation

Purity: 99.87%

Select Batch:

Fiche technique (277 KB) SDS (420 KB)

COA (250 KB) LCMS (127 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Centers for Disease Control and Prevention (CDC). Agranulocytosis associated with cocaine use - four States, March 2008-November 2009.MMWR Morb Mortal Wkly Rep. 2009 Dec 18;58(49):1381-5. [Content Brief]

[2]. Zhu NY, et al. Agranulocytosis after consumption of cocaine adulterated with levamisole. Ann Intern Med. 2009 Feb 17;150(4):287-9. [Content Brief]

[3]. Van Belle H. Alkaline phosphatase. I. Kinetics and inhibition by levamisole of purified isoenzymes from humans. Clin Chem. 1976 Jul;22(7):972-6. [Content Brief]

[4]. Friedlaender MH, et al. The treatment of herpetic reinfection with levamisole. Am J Ophthalmol. 1978 Aug;86(2):245-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO / H₂O	1 mM	4.1537 mL	20.7684 mL	41.5369 mL	103.8422 mL
	5 mM	0.8307 mL	4.1537 mL	8.3074 mL	20.7684 mL
	10 mM	0.4154 mL	2.0768 mL	4.1537 mL	10.3842 mL
	15 mM	0.2769 mL	1.3846 mL	2.7691 mL	6.9228 mL
	20 mM	0.2077 mL	1.0384 mL	2.0768 mL	5.1921 mL
	25 mM	0.1661 mL	0.8307 mL	1.6615 mL	4.1537 mL
	30 mM	0.1385 mL	0.6923 mL	1.3846 mL	3.4614 mL
	40 mM	0.1038 mL	0.5192 mL	1.0384 mL	2.5961 mL
	50 mM	0.0831 mL	0.4154 mL	0.8307 mL	2.0768 mL
DMSO / H₂O	60 mM	0.0692 mL	0.3461 mL	0.6923 mL	1.7307 mL
H₂O	80 mM	0.0519 mL	0.2596 mL	0.5192 mL	1.2980 mL
H₂O	100 mM	0.0415 mL	0.2077 mL	0.4154 mL	1.0384 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Levamisole hydrochloride Related Classifications

Anti-infection
Parasite HSV

Levamisole (hydrochloride) [16595-80-5]

Referência HY-13666-10g

Contactar o distribuidor local :

Marca : MedChemExpress

Contactar o distribuidor local :

Other Forms of Levamisole hydrochloride:

Voir tous les produits spécifiques à Isoform Parasite:

Voir tous les produits spécifiques à Isoform HSV:

Levamisole hydrochloride Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Levamisole hydrochloride Related Classifications

Keywords: