MALT1 inhibitor MI-2 is a MALT1 inhibitor (IC50=5.84 μM). MALT1 inhibitor MI-2 binds directly to MALT1, irreversibly suppresses protease function and is accompanied by NF-κB reporter activity suppression, c-REL nuclear localization inhibition, and NF-κB target gene downregulation. MALT1 inhibitor MI-2 shows nontoxic to animals.
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MALT1 inhibitor MI-2 Chemical Structure
CAS No. : 1047953-91-2
This product is a controlled substance and not for sale in your territory.
Based on 6 publication(s) in Google Scholar
MALT1 inhibitor MI-2 purchased from MedChemExpress. Usage Cited in:
Parasit Vectors. 2018 May 18;11(1):305.
[Abstract]
The A20 protein levels in Jurkat T-cells and Molt-4 T-cells treated with MALT1 inhibitor MI-2 and combined use of MI-2 and ME-49.
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Description
MALT1 inhibitor MI-2 is a MALT1 inhibitor (IC50=5.84 μM). MALT1 inhibitor MI-2 binds directly to MALT1, irreversibly suppresses protease function and is accompanied by NF-κB reporter activity suppression, c-REL nuclear localization inhibition, and NF-κB target gene downregulation. MALT1 inhibitor MI-2 shows nontoxic to animals[1].
IC50 & Target
IC50: 5.84 μM (MALT1)[1]
Cellular Effect
Cell Line
Type
Value
Description
References
OCI-Ly3
IC50
2.6 μM
Compound: MI-2
Antiproliferative activity against human OCI-LY3 cells after 48 hrs by MTT assay
Antiproliferative activity against human OCI-LY3 cells after 48 hrs by MTT assay
[PMID: 26496175]
Toledo
IC50
0.8 μM
Compound: 11; MI-2
Antiproliferative activity against human Toledo cells after 48 hrs by CCK8 assay
Antiproliferative activity against human Toledo cells after 48 hrs by CCK8 assay
[PMID: 29751989]
In Vitro
MALT1 inhibitor MI-2 (1-1000 nM; 48 hours) selectively suppresses MALT1-dependent DLBCL cell lines, and the GI50 in HBL-1, TMD8, OCI-Ly3, and OCI-Ly10 cells is 0.2, 0.5, 0.4, and 0.4 μM, respectively[1]. ?
MALT1 inhibitor MI-2 (62-1000 nM; 24 hours) causes a dose-dependent decrease in MALT1-mediated cleavage[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MALT1 inhibitor MI-2 Related Antibodies
Cell Proliferation Assay[1]
Cell Line:
HBL-1, TMD8, OCI-Ly3, OCI-Ly10 cells
Concentration:
1, 10, 100, 1000 nM
Incubation Time:
48 hours
Result:
The GI50 in HBL-1, TMD8, OCI-Ly3, and OCI-Ly10 cells was 0.2, 0.5, 0.4, and 0.4 µM, respectively.
Western Blot Analysis[1]
Cell Line:
HBL-1 cells
Concentration:
62, 125, 250, 500, 1000 nM
Incubation Time:
24 hours
Result:
Inhibits MALT1 cleavage of CYLD in HBL-1 cells.
In Vivo
MALT1 inhibitor MI-2 (25 mg/kg; i.p.; daily for 14 days) profoundly suppresses the growth of both the TMD8 and HBL-1 ABC-DLBCL xenografts[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Eight-week-old male SCID NOD (bearing HBL-1 and TMD8 cells)[1]
Dosage:
25 mg/kg
Administration:
Intraperitoneal injection; daily for 14 days
Result:
Profoundly suppressed the growth of both the TMD8 and HBL-1 ABC-DLBCL xenografts versus vehicle.
Room temperature in continental US; may vary elsewhere.
Stockage
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
2 years
-20°C
1 year
Solvant et solubilité
In Vitro:
DMSO : ≥ 46 mg/mL (100.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
*"≥" means soluble, but saturation unknown.
Preparing Stock Solutions
ConcentrationSolventMass
1 mg
5 mg
10 mg
1 mM
2.1943 mL
10.9716 mL
21.9433 mL
5 mM
0.4389 mL
2.1943 mL
4.3887 mL
10 mM
0.2194 mL
1.0972 mL
2.1943 mL
View the Complete Stock Solution Preparation Table
*Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2
Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (25.0 mg/mL) to 900 μLCorn oil, and mix evenly.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage
mg/kg
Animal weight (per animal)
g
Dosing volume (per animal)
μL
Number of animals
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO+
%
+
%
Tween-80
+
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
[1]. Fontan L, et al. MALT1 small molecule inhibitors specifically suppress ABC-DLBCL in vitro and in vivo. Cancer Cell. 2012 Dec 11;22(6):812-24.
[Content Brief]
[1]. Fontan L, et al. MALT1 small molecule inhibitors specifically suppress ABC-DLBCL in vitro and in vivo. Cancer Cell. 2012 Dec 11;22(6):812-24.
Complete Stock Solution Preparation Table
*Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent
ConcentrationSolventMass
1 mg
5 mg
10 mg
25 mg
DMSO
1 mM
2.1943 mL
10.9716 mL
21.9433 mL
54.8582 mL
5 mM
0.4389 mL
2.1943 mL
4.3887 mL
10.9716 mL
10 mM
0.2194 mL
1.0972 mL
2.1943 mL
5.4858 mL
15 mM
0.1463 mL
0.7314 mL
1.4629 mL
3.6572 mL
20 mM
0.1097 mL
0.5486 mL
1.0972 mL
2.7429 mL
25 mM
0.0878 mL
0.4389 mL
0.8777 mL
2.1943 mL
30 mM
0.0731 mL
0.3657 mL
0.7314 mL
1.8286 mL
40 mM
0.0549 mL
0.2743 mL
0.5486 mL
1.3715 mL
50 mM
0.0439 mL
0.2194 mL
0.4389 mL
1.0972 mL
60 mM
0.0366 mL
0.1829 mL
0.3657 mL
0.9143 mL
80 mM
0.0274 mL
0.1371 mL
0.2743 mL
0.6857 mL
100 mM
0.0219 mL
0.1097 mL
0.2194 mL
0.5486 mL
MALT1 inhibitor MI-2 Related Classifications
Metabolic Enzyme/ProteaseNF-κB
MALT1
Help & FAQs
Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
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