Mouse Anti-Human IgG4 Fc-HRP

Referência 9200-05

Tamanho : 1.0mL

Marca : Southern Biotech

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Clone HP6025
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-HRP (15H6)
Specificity Human IgG4 Fc
Immunogen Human IgG4 myeloma protein
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 2-9
FLISA – Quality tested
Flow Cytometry – Reported in literature 15,18,19
Immunohistochemistry-Frozen Sections – Reported in literature 10
Immunohistochemistry-Paraffin Sections – Reported in literature 5,7,10,11
Immunocytochemistry – Reported in literature 12
Western Blot – Reported in literature 3,13-16
Microarray – Reported in literature 17
Multiplex – Reported in literature 2,20-22
Purification – Reported in literature 15

RRID Number AB_2796691
Gene ID 3503 (Human)
Gene ID Symbol IGHG4 (Human)
UniProt ID P01861 (Human
UniProt Name IGHG4_HUMAN (Human)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  1. 1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen)
  2. 2. Arnold M, Dechant M, Doxiadis II, Spriewald BM. Prevalence and specificity of immunoglobulin G and immunoglobulin A non-complement-binding anti-HLA alloantibodies in retransplant candidates. Tissue Antigens. 2008;72:60-6. (ELISA, Multiplex)
  3. 3. Bogdanos D, Pusl T, Rust C, Vergani D, Beuers U. Primary biliary cirrhosis following Lactobacillus vaccination for recurrent vaginitis. J Hepatol. 2008;49:466-73. (ELISA, WB)
  4. 4. Forthal DN, Landucci G, Ding H, Kappes JC, Wang A, Thung I, et al. IgG2 inhibits HIV-1 internalization by monocytes, and IgG subclass binding is affected by gp120 glycosylation. AIDS. 2011;25:2099-104. (ELISA)
  5. 5. Jia X, Hu S, Chen J, Qu Z, Liu G, Cui Z, et al. The clinical and immunological features of patients with combined anti-glomerular basement membrane disease and membranous nephropathy. Kidney Int. 2014;85:945-52. (ELISA, IHC-PS)
  6. 6. Xu J, Wang L, Deng C, Hu X, Li R, Chen T, et al. The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection. Parasitol Res. 2014;113:149-55. (ELISA)
  7. 7. Zhang J, Zhao L, Gao Y, Liu M, Li T, Huang Y, et al. A classification of Hashimoto's thyroiditis based on immunohistochemistry for IgG4 and IgG. Thyroid. 2014;24:364-70. (ELISA, IHC-PS)
  8. 8. Westra J, van Assen S, Wilting KR, Land J, Horst G, de Haan A, et al. Rituximab impairs immunoglobulin (Ig)M and IgG (subclass) responses after influenza vaccination in rheumatoid arthritis patients. Clin Exp Immunol. 2014;178:40-7. (ELISA)
  9. 9. Seitz-Polski B, Payré C, Ambrosetti D, Albano L, Cassuto-Viguier E, Berguignat M, et al. Prediction of membranous nephropathy recurrence after transplantation by monitoring of anti-PLA2R1 (M-type phospholipase A2 receptor) autoantibodies: a case series of 15 patients. Nephrol Dial Transplant. 2014;29:2334-42. (ELISA)
  10. 10. Detlefsen S, Bräsen JH, Zamboni G, Capelli P, Klöppel G. Deposition of complement C3c, immunoglobulin (Ig)G4 and IgG at the basement membrane of pancreatic ducts and acini in autoimmune pancreatitis. Histopathology. 2010;57:825-35. (IHC-FS, IHC-PS)
  11. 11. Qu Z, Liu G, Li J, Wu L, Tan Y, Zheng X, et al. Absence of glomerular IgG4 deposition in patients with membranous nephropathy may indicate malignancy. Nephrol Dial Transplant. 2012;27:1931-7. (IHC-PS)
  12. 12. de Bruin A, Caldelari R, Williamson L, Suter MM, Hunziker T, Wyder M, et al. Plakoglobin-dependent disruption of the desmosomal plaque in pemphigus vulgaris. Exp Dermatol. 2007;16:468-75. (ICC)
  13. 13. Müller E, Kernland K, Caldelari R, Wyder M, Balmer V, Hunziker T, et al. Unusual pemphigus phenotype in the presence of a Dsg1 and Dsg3 autoantibody profile. J Invest Dermatol. 2002;118:551-5. (WB)
  14. 14. Bogdanos D, Pares A, Baum H, Caballeria L, Rigopoulou EI, Ma Y, et al. Disease-specific cross-reactivity between mimicking peptides of heat shock protein of Mycobacterium gordonae and dominant epitope of E2 subunit of pyruvate dehydrogenase is common in Spanish but not British patients with primary biliary cirrhosis. J Autoimmun. 2004;22:353-62. (WB)
  15. 15. Keen J, Serghides L, Ayi K, Patel SN, Ayisi J, van Eijk A, et al. HIV impairs opsonic phagocytic clearance of pregnancy-associated malaria parasites. PLoS Med. 2007;4(5):e181. (WB, FC, Purificaton)
  16. 16. Parcina M, Miranda-Garcia MA, Durlanik S, Ziegler S, Over B, Georg P, et al. Pathogen-triggered activation of plasmacytoid dendritic cells induces IL-10-producing B cells in response to Staphylococcus aureus. J Immunol. 2013;190:1591-602. (WB)
  17. 17. Savilahti EM, Rantanen V, Lin JS, Karinen S, Saarinen KM, Goldis M, et al. Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes. J Allergy Clin Immunol. 2010;125:1315-21. (Microarray)
  18. 18. Johansson SE, Hejdeman B, Hinkula J, Johansson MH, Romagné F, Wahren B, et al. NK cell activation by KIR-binding antibody 1-7F9 and response to HIV-infected autologous cells in viremic and controller HIV-infected patients. Clin Immunol. 2010;134:158-68. (FC)
  19. 19. Gao B, Moore C, Porcheray F, Rong C, Abidoglu C, DeVito J, et al. Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss. Am J Transplant. 2014;14:1581-91. (FC)
  20. 20. Lowe D, Higgins R, Zehnder D, Briggs DC. Significant IgG subclass heterogeneity in HLA-specific antibodies: Implications for pathogenicity, prognosis, and the rejection response. Hum Immunol. 2013;74:666-72. (Multiplex)
  21. 21. Arnold M, Ntokou I, Doxiadis II, Spriewald BM, Boletis JN, Iniotaki AG. Donor-specific HLA antibodies: evaluating the risk for graft loss in renal transplant recipients with isotype switch from complement fixing IgG1/IgG3 to noncomplement fixing IgG2/IgG4 anti-HLA alloantibodies. Transpl Int. 2014;27:253-61. (Multiplex)
  22. 22. Schaub S, Hönger G, Koller MT, Liwski R, Amico P. Determinants of C1q binding in the single antigen bead assay. Transplantation. 2014;98:387-93. (Multiplex)

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0203-SC
 50ml