NHS-activated SepFast 4HF
Referência 330101-5ML
Tamanho : 5ml
Marca : BioToolomics
Introduction
The base matrix is activated through a long hydrophilic spacer arm with N-hydroxysuccinimide at the end that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds.
NHS (N-hydroxysuccinimide)-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
NHS-activated SepFast media forms a chemically stable amide bond with ligands containing primary amine groups. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.
Coupling manual is supplied with each product.
Characteristics of NHS-activated SepFast media
Group to be coupled | -NH2 |
Coupling conditions | pH 6 – 9, 4oC to 30oC, 2 - 16 h, organic solvents possible |
Matrix | SepFast 4HF: highly cross-linked 4% agarose |
Particle size | 50 – 150 μm |
Activation level | 5 - 19 μmol active group / ml medium |
pH stability | 3 -13 (ligand dependent) |
Chemical stability | Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand |
Storage | 4oC – 8oC |