Images
Western Blot
Application

Western Blot

Western blot analysis of recombinant protein with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940). Lane 1& 2 each contain 100 ng recombinant PDCD4. Lanes 3& 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining of formalin-fixed paraffin-embedded human breast epithelial cell with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940).

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDCD4.

    Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding S457 of human PDCD4.

    Host

    Rabbit

    Theoretical MW (kDa)

    52

    Reactivity

    Human, Mouse, Rat, Xenopus

    Specificity

    This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.

    Form

    Liquid

    Purification

    Immunoaffinity purification

    Recommend Usage

    ELISA (1:400000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (21.25-2.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of recombinant protein with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940). Lane 1& 2 each contain 100 ng recombinant PDCD4. Lanes 3& 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining of formalin-fixed paraffin-embedded human breast epithelial cell with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940).

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — PDCD4

    Entrez GeneID

    27250

    Gene Name

    PDCD4

    Gene Alias

    H731, MGC33046, MGC33047

    Gene Description

    programmed cell death 4 (neoplastic transformation inhibitor)

    Omim ID

    608610

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq

    Other Designations

    OTTHUMP00000020483|nuclear antigen H731|programmed cell death 4

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