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> Rat IgM (mu chain) antibody

Rat IgM (mu chain) antibody

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Referência orb347851-2mg

Tamanho : 2mg

Marca : Biorbyt

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    Rat IgM (mu chain) antibody

    Catalog Number: orb347851

    Catalog Numberorb347851
    CategoryAntibodies
    DescriptionRat IgM (mu chain) antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRat
    IsotypeIgG
    ImmunogenRat IgM whole molecule
    Concentration2.4 mg/mL
    Dilution rangeELISA: 1:50,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
    Form/AppearanceLiquid (sterile filtered)
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
    ConjugationUnconjugated
    StorageStore vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesRabbit Anti Rat IgM (mu chain) Antibody, Rabbit An
    NoteFor research use only
    Application notesAnti-Rat IgM mu heavy chain antibody is tested by western blot and suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
    Expiration Date12 months from date of receipt.
    Rat IgM (mu chain) antibody

    Western Blot of Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n orb347851). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n orb347851) 1:2000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.