Tap1 Polyclonal Antibody
Referência bs-2789R-20ul
Tamanho : 20ul
Marca : Bioss
Tap1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
Overview | |
Catalog # | bs-2789R |
Product Name | Tap1 Polyclonal Antibody |
Applications | WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
Reactivity | Human, Pig |
Predicted Reactivity | Mouse, Rat, Cow, Rabbit |
Specifications | |
Conjugation | Unconjugated |
Host | Rabbit |
Source | KLH conjugated synthetic peptide derived from human Tap1/ABCB2 |
Immunogen Range | 501-600/808 |
Clonality | Polyclonal |
Isotype | IgG |
Concentration | 1ug/ul |
Purification | Purified by Protein A. |
Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target | |
Gene ID | 6890 |
Swiss Prot | Q03518 |
Subcellular location | Cytoplasm |
Synonyms | APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*12N; Antigen peptide transporter 1; ATP-binding cassette sub-family B member 2; Peptide supply factor 1; Peptide transporter PSF1; Peptide transporter TAP1; Peptide transporter involved in antigen processing 1; Really interesting new gene 4 protein; TAP1; Y3 |
Background | Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-1 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. |
Application Dilution | |
WB | 1:300-5000 |
ELISA | 1:500-1000 |
FCM | 1:20-100 |
IHC-P | 1:200-400 |
IHC-F | 1:100-500 |
IF(IHC-P) | 1:50-200 |
IF(IHC-F) | 1:50-200 |
IF(ICC) | 1:50-200 |