BPS Bioscience
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BPS offers one of the largest selections of unique recombinant proteins and assay kits for epigenetic studies, immune checkpoints, metabolism and cell signaling pathways to improve human health.
Cloning and Expression: BPS Bioscience's Molecular Biology Services team can help you achieve any molecular biology research goal you are trying to achieve. Our gene cloning and site-directed mutagenesis services are often the first step in custom protein expression, analytic development, and custom cell line development projects.
Purification and Protein Modification: Choose BPS Bioscience for your protein, reduction, purification and modification needs. Using our cloning and expression services, the constructs can be optimized for high purity, high performance protein expression. We are also specialized in protein labeling to provide improved products for high quality testing.
Cell line development: Let our experts help you reduce time and complexity with our stable cell line development services. We produce customized cell lines for protein production and compound screening. Our specialty in reporter assays for immune control pathways offers great opportunities for research on co-immunotherapies.
Test Development and Screening Services: Evaluate key compounds with our biochemical and cell screening services. Save time by allowing us to filter your compounds of interest against our unique test panels or to determine IC50 values with our portfolio of hundreds of tests so you can focus on advancing your discovery program of drugs.
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Website: bpsbioscience.com
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Blocking buffers
The surfaces of the ELISA plates or Western blot membranes are optimized for efficient binding of proteins and antibodies. This allows for optimum dosages with a high density of capture antibodies or capture molecules on the surfaces. As a result of this surface optimization, many other proteins or peptides and other molecules in the sample can also attach to the surface.
Without blocking the surface, antibodies, tracers, analytes or other components of the sample would bind to the surface. This can lead to false results or high background noise which must be avoided in order to obtain reliable dosages.
Blocking is necessary to saturate the free bonding capacities of the surfaces. This saturation can be obtained by covering the surface of a dense layer of molecules without spaces in this layer.
We offer different types of blockers with different formulations and blocking characteristics. The main types of blockers are blocking buffers based on BSA or casein. There are also other protein-free blocking buffers.
