Abbexa

Nucleic acids extraction

Reagents and instruments for immunology, cell biology and molecular biology.

Abbexa is a dedicated worldwide supplier of biological tools for the life science, pharmaceutical and biotechnology research and development sectors. Abbexa provides the scientific community with primary antibodies, secondary antibodies, proteins, ELISA kits and enzymes as well as other kits and tools designed for use in research. Abbexa also offers custom protein, peptide and antibody production services to meet customer requirements.

Abbexa is committed to providing scientists and researchers with the best customer experience and high-quality products.

Abbexa’s mission is to offer unrivalled expertise and outstanding quality laboratory consumable products, striving to consistently improve. Please do get in touch with any comments regarding the website, service, products, or anything else.

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Website : www.abbexa.com

Antibodies for Cancer Research

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ELISA Kits for Accurate Detection

Primary Antibodies

Nucleic acid extraction methods are essential to molecular biology and are commonly used in many medical and biological science applications.

Nucleic acid extraction can be divided into 3 steps, which can be optimized according to the type of sample and the downstream applications for which the nucleic acids will be used. These steps are as follows:

1) Rupture of tissue and cell structures

2) Removal of proteins, lipids and other contaminants from nucleic acids

3) Transfer the nucleic acids to water or a buffer solution that will preserve them without interfering with subsequent work.

Nucleic acid extraction methods can be classified into two different types :

Solution methods (such as the phenol-chloroform method)
Methods based on the solid phase (such as magnetic beads or centrifugation columns).

Solid-phase extraction methods work by causing nucleic acids to bind to solid supports, such as magnetic beads coated with silica or other materials. The beads (or other supports) are then washed with alcohol to remove contaminants. The support is then washed with a liquid that makes the nucleic acids soluble again, which releases the DNA from the support in a process known as elution. A simple method is to add a solution containing a chaotrope to the crude nucleic acid extract. Chaotropic agents are molecules that disrupt the hydrogen bonding network between water molecules. In the presence of chaotropes, nucleic acids are previously much less soluble and bind to glass, silica-covered magnetic beads and other solid supports - allowing them to be easily separated from contaminants. Solid-phase extraction methods may also depend on selective binding of DNA and RNA to ion exchange resins or other chemicals.

Nucleic acid gel stain

RNA

DNA