Reagents and instruments for immunology, cell biology and molecular biology.
 

Abbexa

 

Abbexa is a dedicated worldwide supplier of biological tools for the life science, pharmaceutical and biotechnology research and development sectors. Abbexa provides the scientific community with primary antibodies, secondary antibodies, proteins, ELISA kits and enzymes as well as other kits and tools designed for use in research. Abbexa also offers custom protein, peptide and antibody production services to meet customer requirements.

Abbexa is committed to providing scientists and researchers with the best customer experience and high-quality products.

Abbexa’s mission is to offer unrivalled expertise and outstanding quality laboratory consumable products, striving to consistently improve. Please do get in touch with any comments regarding the website, service, products, or anything else.

 

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Website : www.abbexa.com
 
 
> Abbexa > Products for research > Molecular Biology Tools > PCR products > Quantitative RT-PCR - qRT-PCR > RT-qPCR SYBR Green Mixes > Universal SYBR Green Mixes for RT-qPCR > Two-Step RT-qPCR mixes - SYBR Green
Two-Step RT-qPCR mixes - SYBR Green

Two-Step RT-qPCR mixes - SYBR Green


The two-step RT-qPCR involves two distinct reactions, starting with first strand cDNA synthesis (reverse transcription-RT) and then amplifying part of the resulting cDNA by quantitative PCR in a separate tube . Therefore, two-step RT-qPCR is useful for detecting multiple genes in a single RNA sample. The separation of the RT and quantitative PCR reactions makes it possible to optimize the reaction conditions for each step as well as the flexibility with priming by reverse transcription (oligo (dT) primers, random hexamers or gene-specific primers) and PCR DNA polymerase and PCR components). Compared to one-step RT-qPCR, the disadvantages of two-step RT-qPCR include several steps for extended workflow, additional handling and processing of the sample, and increase the risk of contamination and variation results.

two-step-RT-PCR


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Cat#
Description
Cond.
Price Bef. VAT
abx098035-50rxns×20ul(RTSystems)/300rxns×20ul(qPCRSystems)
Green Two-Step qRT-PCR SuperMix
 50rxns×20ul(RTSystems)/300rxns×20ul(qPCRSystems) 
abx098036-20rxns×20ul(RTSystems)/500rxns×20ul(qPCRSystems)
Green miRNA Two-Step qRT-PCR SuperMix
 20rxns×20ul(RTSystems)/500rxns×20ul(qPCRSystems) 
abx098037-50rxns×20ul(RTSystems)/300rxns×20ul(qPCRSystems)
Green Two-Step qRT-PCR SuperMix (GC Rich)
 50rxns×20ul(RTSystems)/300rxns×20ul(qPCRSystems) 
abx461007-500rxns
RT-qPCR Extraction Control Red
 500rxns 
abx461077-500rxns
RT-qPCR Extraction Control Orange
 500rxns 
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