Reagents and instruments for immunology, cell biology and molecular biology.
 

Abbexa

 

Abbexa is a dedicated worldwide supplier of biological tools for the life science, pharmaceutical and biotechnology research and development sectors. Abbexa provides the scientific community with primary antibodies, secondary antibodies, proteins, ELISA kits and enzymes as well as other kits and tools designed for use in research. Abbexa also offers custom protein, peptide and antibody production services to meet customer requirements.

Abbexa is committed to providing scientists and researchers with the best customer experience and high-quality products.

Abbexa’s mission is to offer unrivalled expertise and outstanding quality laboratory consumable products, striving to consistently improve. Please do get in touch with any comments regarding the website, service, products, or anything else.

 

Learn more :

 

 

  

  

 

 

         

   

  

Antibodies for Cancer Research

     

Cell Biology Antibodies

    

ELISA Kits for Accurate Detection

   Primary Antibodies
             

 

 

Website : www.abbexa.com
 
 
> Abbexa > Products for research > Molecular Biology Tools > Nucleic acids extraction > RNA extraction > Specific RNA type > Extraction and purification of total RNA > Extraction and purification of total RNA - Animal > Total RNA extraction - Precipitation
Total RNA extraction - Precipitation

Total RNA extraction - Precipitation


The most common organic extraction is the method using phenol-chloroform solution
Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation.
Organic extraction involves the addition of and incubation in multiple different chemical solutions; including a lysis step, a phenol chloroform extraction, an ethanol precipitation, and washing steps.
Samples are homogenized in a phenol-containing solution and then centrifuged after chloroform addition. During centrifugation, the sample separates into three phases: an upper aqueous phase which contains RNA, an interface containing DNA, and a lower organic phase containing proteins and lipids.
The advantage of the phenol-chloroform method is the simplicity of the protocol, the rapid denaturation of nucleases and the stabilization of nucleic acids. The main disadvantage is the use of toxic chemicals phenol and chloroform, and there is an increased risk of contamination due to transferring the DNA between multiple tubes.

Search result : 11 product found

Refine your search :

RUOCE / IVD
  • Biochemicals 11
APPLY FILTERS
REINITIALIZE


Cat#
Description
Cond.
Price Bef. VAT
abx089253-100ml
Tetrazole
 100ml 
abx082213-250mg
Thiazolyl Blue Tetrazolium Bromide
 250mg 
abx082549-250mg
Thiazolyl Blue Tetrazolium Bromide
 250mg 
abx082368-100mg
Nitrotetrazoliumbluechloride
 100mg 
abx082368-1000mg
Nitrotetrazoliumbluechloride
 1000mg 
abx082536-1000mg
Nitrotetrazoliumbluechloride
 1000mg 
abx082536-100mg
Nitrotetrazoliumbluechloride
 100mg 
    ‹
    ›