Abbexa
Abbexa is a dedicated worldwide supplier of biological tools for the life science, pharmaceutical and biotechnology research and development sectors. Abbexa provides the scientific community with primary antibodies, secondary antibodies, proteins, ELISA kits and enzymes as well as other kits and tools designed for use in research. Abbexa also offers custom protein, peptide and antibody production services to meet customer requirements. Abbexa is committed to providing scientists and researchers with the best customer experience and high-quality products. Abbexa’s mission is to offer unrivalled expertise and outstanding quality laboratory consumable products, striving to consistently improve. Please do get in touch with any comments regarding the website, service, products, or anything else.
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F(ab) fragmented secondary antibodies
Fab and F(ab')2 fragments are two types of fragmented secondary antibodies that are commonly used in various applications. Here are some key points about F(ab) fragments:
- F(ab) fragments are monovalent antibody fragments that contain only one antigen-binding Fab portion and lack the Fc region. They are generated by papain digestion of whole IgG antibodies to remove the Fc region and the entire hinge region.
- F(ab) fragments have a molecular weight of about 50 kDa, which is smaller than whole IgG antibodies and F(ab')2 fragments. This allows for better penetration into tissues and reduces nonspecific binding to Fc receptors on live cells or to Protein A/G.
- F(ab) fragments are useful for blocking endogenous immunoglobulins on cells, tissues, or other surfaces, and for blocking the exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species. They can also be used to Fab-label primary antibodies prior to incubation with the experimental sample.
- F(ab) fragments are monovalent and have lower avidity than F(ab')2 fragments, which may affect their sensitivity and specificity in certain applications.
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