ELISA kits are enzyme-linked immunosorbent assays designed for the quick detection and quantitation of target proteins, antibodies, and other soluble targets of interest.
Type of ELISA kits
Sandwich ELISA for quantitative protein detection
Sandwich ELISA kits utilize matched antibody pairs for a "sandwich-based" quantitative detection of cytokines, growth factors, receptors and other proteins.
Indirect ELISA for detection of Antibody Response to Pathogens
An indirect Enzyme-Linked Immunosorbent Assay (ELISA) is a common biochemical technique that is most suitable for determining total antibody concentrations in a sample. This method is commonly utilized to diagnose infection and to quantify antibodies against the invading antigen.
Phosphorylation ELISA - Semi-Quantitative, Sandwich-based Detection of Phosphorylated Proteins in Lysates
Phosphorylation ELISA kits are sandwich-based ELISAs specificly made for measuring phosphorylated proteins, such as MAPK, AKT, EGFR, STAT, mTOR, and other signaling molecules. This allows the researcher to screen numerous different cell lysates without performing Western Blot analyses. For normalization purposes, these kits can also measure phosphorylated protein and pan protein in one experiment.
Competitive ELISA (EIA) - Quantitative, Competition-based Detection of Soluble Factors
Competitive ELISA kits utilize the principle of competitive enzyme immunoassay (EIA), in which the target protein and a biotin-conjugated peptide bind competitively to a capture antibody. This method is effective for detecting peptide hormones and other molecules for which no antibody pair has been developed.
Cell-based ELISA - Semi-Quantitative, Direct Detection of Phosphorylated Proteins in Adherent Cell Lines
Cell-Based ELISA kits utilize the direct design principle to measure the relative amount of protein phosphorylation and monitor the effect of various treatments, inhibitors, or activators in cultured cell lines.
